Welcome to the PathoSense Dry Lab installation guide!

Here you'll find everything needed to master our data transfer and cloud platform procedures.

What You'll Find Here:

  • Materials List - Complete inventory of materials for establishing the PathoSense cloudlink.
  • Dry Lab Learning Path - Step-by-step training to install necessary software and establish a secure cloudlink to transfer data from your lab to PathoSense.
  • Test Data - Download test files to ensure your cloudlink connection with PathoSense is functioning correctly.

Getting Started:

  1. Review the materials list to ensure your lab has all necessary digital components
  2. Complete each module in the learning path in sequential order
  3. Successfully pass all quizzes to document your proficiency

Please complete the entire Dry Lab Learning Path before scheduling your follow-up meeting.

 Materials Dry Lab

Material List ✅

You can find the complete list of all materials needed for the PathoSense Dry Lab here:

Material List Dry Lab

 Test data Dry Lab

Here, you can download test data to verify that MinKNOW is installed correctly and to test the basecalling process. All instructions on how to use this test data are provided in the instructional video "DRY LAB II: MinKNOW." This video will guide you through the steps to ensure proper setup and functionality.

First go through the Learning Path Dry Lab

 Learning Path Dry Lab

Dry lab 1: Ubuntu

Instructional movie

This procedure explains how to make a bootable USB stick and install Ubuntu 22.04 with the
required settings for a smooth PathoSense set up.
It is recommended to follow the installation along with the SOP and the provided
video presentations.

Dry lab 1: Ubuntu

SOP

This procedure explains how to make a bootable USB stick and install Ubuntu 22.04 with the
required settings for a smooth PathoSense set up.
It is recommended to follow the installation along with the SOP and the provided
video presentations.

Can I install the newest Ubuntu version?

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Dry lab 2: MinKNOW

Instructional movie

This procedure explains how to install and test the MinKNOW software to enable sequencing
and GPU basecalling.
Basecalling is the process of converting the raw sequencing signal from the sequencer and
converting that to the actual DNA sequence. This is computationally very intensive and
requires the GPU to be correctly configured.

Dry lab 2: MinKNOW

SOP

This procedure explains how to install and test the MinKNOW software to enable sequencing
and GPU basecalling.
Basecalling is the process of converting the raw sequencing signal from the sequencer and
converting that to the actual DNA sequence. This is computationally very intensive and
requires the GPU to be correctly configured.

What is basecalling?

 

 

 

Dry lab 3: CloudLink

Instructional movie

This procedure explains how to install and test CloudLInk. The CloudLink enables data
transfer of sequencing run data to the Pathosense Cloud for subsequent data analysis. This
way, manual data transfers are avoided.

Dry lab 3: CloudLink

SOP

This procedure explains how to install and test CloudLInk. The CloudLink enables data
transfer of sequencing run data to the Pathosense Cloud for subsequent data analysis. This
way, manual data transfers are avoided.

How long do you need to store the data?

How long does PathoSense store the data?

What to do when you remove the data from the folder (cut-paste) and add the data back to the folder?

Where can I see if the data was successfully sent?

Why is automatic data transfer important?